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Genetic manipulation of receptor expression and function

  • 240 Pages
  • 2.84 MB
  • English

Wiley-Liss , New York
Cell receptors, Genetic engineering, Gene targeting, Cell receptors -- Research -- Method
Statementedited by Domenico Accili
SeriesReceptor biochemistry and methodology, Receptor biochemistry and methodology (Unnumbered)
ContributionsAccili, Domenico
LC ClassificationsQH603.C43 G47 2000
The Physical Object
Paginationxii, 240 p., [4] p. of plates :
ID Numbers
Open LibraryOL16973476M
ISBN 100471350575, 0471350567
LC Control Number99042182

Genetic Manipulation of Receptor Expression and Function: Medicine & Health Science Books @ By providing the necessary conceptual framework of transgenic and knockout technologies, Genetic Manipulation of Receptor Expression and Function offers a concise and easily accessible analysis for researchers.

The material reviews the numerous advances in the field of transgenic technology allowing researchers to alter expression and function in the mouse genome. Domenico Accili is the author of Genetic Manipulation of Receptor Expression and Function, published by : Domenico Accili.

ISBN: OCLC Number: Description: xii, pages: illustrations ; 26 cm. Contents: Ch. Transgenic rescue of mutant phenotypes using large DNA fragments --Ch. oral characterization of transgenic and knockout mice --Ch. c analysis of receptor phenotypes --Ch. alization of cell lines from.

Read Book PDF Online Here ?book=PDF Genetic Manipulation of Receptor Expression and Function PDF Book Free.

Estrogen receptors (ER) include ER alpha, ER beta and new membrane receptor G protein-coupled receptor 30 (GPR30). Estrogen receptors are key receptors to maintain ovarian granulosa cell differentiation, follicle and oocyte growth and development, and ovulation by: 4.

The book shows how these heritable mechanisms allow individual cells to establish stable and unique patterns of gene expression that can be passed through cell divisions without DNA mutations, thereby establishing how different heritable patterns of gene regulation control cell differentiation and organogenesis, resulting in a distinct human.

The ligand-receptor complex moves into the nucleus, then binds to specific regions of the DNA and promotes the production of mRNA from specific genes (Figure 2). Internal receptors can directly influence gene expression (how much of a specific protein is produced from a gene) without having to pass the signal on to other receptors or : Lisa Bartee, Walter Shriner, Catherine Creech.

The receptors are divided into two subtypes: calcitonin receptor (CTR), which binds CT, and calcitonin-like receptor (CLR), for which the endogenous ligand is unknown.

CGRP, AMY, AM1, AM2, and AM5 are distinguished by the co-expression of CTR or CLR with receptor activity modifying proteins. CT has hypocalcemic activity. Targeted therapies are used in patients with a set gene expression pattern.

A targeted therapy that prevents the activation of the estrogen receptor in breast cancer would be beneficial to which type of patient. patients who express the EGFR receptor in normal cells b.

patients with a mutation that inactivates the estrogen receptor. Clearly, the expression of the wrong protein dramatically alters cell function and contributes to the development of cancer.

New Drugs to Combat Cancer: Targeted Therapies Scientists are using what is known about the regulation of gene expression in disease states, including cancer, to develop new ways to treat and prevent disease development.

CRYPTOCHROME FUNCTIONS. The functions of Arabidopsis cryptochromes have been revealed by genetic studies of the loss-of-function mutants of the CRY1 or CRY2 genes and gain-of-function transgenic plants overexpressing either the wild type or mutant CRY1 and CRY2 genes.

Those studies demonstrate that Arabidopsis CRY1 and CRY2 primarily mediate blue-light stimulation Cited by: The insulin receptor gene encodes an evolutionarily conserved signaling protein with a wide spectrum of functions in metazoan development.

The insulin signaling pathway plays key roles in processes such as metabolic regulation, growth control, and neuronal function. Misregulation of the pathway features in diabetes, cancer, and neurodegenerative diseases, making it an important target for Author: Sandhya Payankaulam, Ana-Maria Raicu, David N.


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Transcriptional expression of A. hetaohei neuropeptides and neuropeptide receptor genes in both male and female tissues were analyzed by Real-time quantitative polymerase chain reaction (RT-qPCR). Gene-specific primers were designed using Primer (Premier Biosoft, ) and are listed in Supplementary Table S3 and : Feifei Li, Xing Zhao, Shuying Zhu, Tao Wang, Tianfeng Li, Tracy Woolfley, Guanghui Tang.

Molecular Mechanisms of β 2-Adrenergic Receptor Function and Regulation. Dennis W. McGraw and Stephen B. Liggett expression of phospholipase C is increased, the functions of G q-coupled constrictive receptors are enhanced, any genetic variation that alters expression, Cited by:   Lamina propria DCs, but not splenic DCs, express high levels of TLR5 protein.

We first examined the gene expression of murine Tlr5 among different tissues including the spleen, liver, thymus and intestine by quantitative real-time 5 mRNA was found to be highly expressed only in the intestine (Figure 1a).To determine the Tlr5 gene expression pattern along the whole intestinal tract Cited by: Human nervous system - Human nervous system - Receptors: Receptors are biological transducers that convert energy from both external and internal environments into electrical impulses.

They may be massed together to form a sense organ, such as the eye or ear, or they may be scattered, as are those of the skin and viscera.

Receptors are connected to the central nervous system by afferent nerve. Acting principally to control patterns of gene expression, nuclear receptors play vital roles during embryonic development and in the regulation of metabolic and reproductive functions in adult life, which proves this superfamily of ligand-activated transcription factors to be a crucial part of biological life.

The androgen receptor displays in many respects a unique mechanism of transcriptional activation compared with other described receptors. Most of its transcriptional activity is mediated through its N-terminal AF-1 domain (84), which contains homopolymer stretches of glutamine and glycine amino acids (85).Cited by:   Seven-transmembrane-spanning receptors and heart function it seems that modest levels of β 2-AR expression can improve heart function.

these data suggest that genetic manipulation and Cited by: In a previous study, we observed that the TLR4/NF-κB axis is critical for ABCB1 expression in taxol-resistant ovarian cancer cells. We identified here that the transcription factor NF-κB is a candidate driver of DEGs (gene targets: 65/ = %), but the results of our transcriptomic assay showed that NF-κB covered a smaller number of gene.

Genetic engineering, also called genetic modification or genetic manipulation, is the direct manipulation of an organism's genes using is a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel DNA is obtained by either isolating and copying the genetic.

Apolipoprotein E (APOE) is a protein involved in the metabolism of fats in the is implicated in Alzheimer's disease and cardiovascular disease.

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APOE belongs to a family of fat-binding proteins called the circulation, it is present as part of several classes of lipoprotein particles, including chylomicron remnants, VLDL, IDL, and some s: APOE, AD2, APO-E, LDLCQ5. Ensembl ENSG ENSMUSG UniProt P P RefSeq (mRNA) NM_ NM_ NM_ NM_ RefSeq (protein) NP_ NP_ NP_ NP_ Location (UCSC) Chr – Mb Chr – Mb PubMed search Wikidata View/Edit Human View/Edit Mouse Leukemia inhibitory factor, or LIF, is an interleukin Aliases: LIF, CDF, DIA, HILDA, MLPLI.

The superfamily of G protein-coupled receptors (GPCRs) includes at least seven-transmembrane receptors that participate in diverse physiological and pathological functions Cited by: The receptor gene has multiple potential proximal regulatory elements. In general estrogen up-regulates expression and progesterone is inhibitory.

The sequence of estrogen and progesterone treatment over several days followed by progesterone withdrawal, mimicking pregnancy levels of these sex steroids, up-regulates oxytocin receptor expression in brain regions critical for maternal behavior.

Regulation of gene expression gives control over the timing, location, and amount of a given gene product (protein or ncRNA) present in a cell and can have a profound effect on the cellular structure and function.

Regulation of gene expression is the basis for cellular differentiation, development, morphogenesis and the versatility and adaptability of any organism. Gene regulation may also serve. Nuclear hormone receptor.

The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription.

IL-1ra functions as a competitive inhibitor of IL-1 receptor in vivo and in vitro. It counteracts the effects of both IL-1α and IL-1β.

Description Genetic manipulation of receptor expression and function PDF

Upon binding of IL-1ra, the IL-1 receptor does not transmit a signal to the cell. IL-1ra inhibits the release of both IL-1α and IL-1β, IL-2 secretion, cell surface IL-2 receptor ro: IPR   Ihog proteins essential for Hh transduction and target gene expression.

As loss of Wg expression and naked cuticle in each segment of the developing embryo can be caused by effects other than simple loss of Hh signaling, we further assessed the function of ihog/boi by examining expression of Hh target genes in the wing imaginal disc, the precursor of the adult by:.

a, Hana3A cell luciferase assays of unique human odorant receptors; the concentration of androstenone used was 30 s and coloured bars indicate different OR families. b, Allele Cited by:   The AR gene provides instructions for making a protein called an androgen receptor.

Androgens are hormones (such as testosterone) that are important for normal male sexual development before birth and during puberty. Androgen receptors allow. The genetic basis of organismal expression is ingrained in the biological sciences as a consensual truth, a belief by which we frame our reference for health and disease.

Hence the notion that susceptibility to certain illnesses or the expression of aberrant behavior is generally linked to genetic lineage and, on occasions, spontaneous mutations.